Perform and assist with the conduct of experiments in a laboratory investigating mechanisms of metastasis in childhood cancers. Work within a team that includes principal investigator, highly experienced senior scientists, post docs and graduate students. Duties will include cell culture, cloning, virus generation and cell transformation, as well as some animal work. Must have molecular biology experience. Must be able to work some evening and weekends in an on-call and as needed rotation.

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PRINCIPAL DUTIES AND RESPONSIBILITIES:

Assist with conducting research studies and laboratory analysis in studies of pediatric cancer in collaboration with principal investigator and laboratory manager.
• Process clinical research specimens received from clinical trial participants.
• Monitors animals for tumor growth and doses experimental medications.
• Maintains cultures of various cell lines. Performs in-vitro experiments with analyses that include ELISA, western blot, microscopy, flow cytometry and library preparation for sequencing.
• Generates plasmids for producing lentiviruses to manipulate gene expression in cell culture (shRNA, CRISPR).
• Produces viral preparations, transduces cell cultures, and characterizes the effects of these manipulations.
• Collects and analyzes data in accordance with appropriate statistical procedures, and prepares reports, inclusive of graphs, tables, and photographs, of the results of studies.
• Assists in the preparation of scientific publications and grant proposals with regard to a specific area of research.
• Performs miscellaneous related duties as required.

KNOWLEDGE , SKILLS AND ABILITIES REQUIRED

• Bachelor of Science degree with 6 months to two years of laboratory research experience OR certification and experience may be considered in lieu of formal education.
• Must be able to use research methods in generating and collecting data and analyzing the data for report preparation.

Minimum Physical Requirements:

• Percent of time spent on: 50% Conducting research studies 30% Collecting and analyzing data 10% Assist with preparing position papers, publications and grant proposals 05% Resource person for other investigators 05% Mentoring less experienced research personnel Minimum physical requirements: 100% Kneeling/reaching 100% Finger dexterity 100% Talking hearing / seeing 33% Lifting/carrying weight 0-20 pounds for 1-5 minutes
• The above list of duties is intended to describe the general nature and level of work performed by individuals assigned to this classification. It is not to be construed as an exhaustive list of duties performed by the individual so classified, nor is it intended to limit or modify the right of any supervisor to assign, direct, and control the work of employees under his/her supervision.