Job Title: Laboratory Technician
Location: Bethesda MD 20892
Duration: 3 Years to Permanent
Shift: 40 Hours/Week (Mon to Fri)
Onsite: Yes
 

Job Description:

• Laboratory Technician for Viral Immunology and Intravital Imaging Section (VIIIS) to perform a variety of laboratory techniques and procedures, especially managing mice colonies.           

Min Education:

• Master's             

• Master's degree in a cell biology, neurobiology, or related discipline

• Strong scientific communications skills, both oral and written.
• Team player in a large research institutes.
• Experience with human iPSC culture and automated platforms
• Experience in scripting/coding to create, test, and perform new methods in automated cell culture platforms (basic Venus-based method scripting for Hamilton robot would be very much appreciated)
• Knowledge of proper laboratory disposal methods and procedures
• Strong communications skills, both oral and written
• Excellent analytical, organizational and time management skills

• Biology
• Miscellaneous Biology
• Biochemical Sciences
• Neuroscience

• Work with staff on performing various experiments and procedures related to differentiation and phenotyping of induced pluripotent stem cell lines,
• Work with staff to provide insight into the effects of ADRD-associated mutations on molecular readouts in cell culture.
• Work with staff to characterize genetic pathways for Alzheimer's disease and related dementia (ADRD) using human cell-based models
• Work with staff to apply published protocols for cell differentiation to IPSC lines, microscopy, and molecular analysis.
• Program, operate, and implement Client methods on liquid handling platforms (Hamilton, Tecan)
• Work with staff on performing large-scale iPSC cell culture experiments on liquid handling platforms.
• Support other research projects

• Collaborate with other team members to carry out large-scale cell culture protocols using high throughout robotic systems
• Perform standard immunological techniques, including western blotting and immunostaining

• Maintain laboratory equipment
• Operate laboratory equipment to analyze samples
• Analyze laboratory findings
• Train staff on the proper usage of laboratory instruments and equipment.
• Provide assistance to laboratory staff in designing experiments, using instrumentation, data acquisition and analysis, and preparing figures for publication.
• Work as a team to provide upkeep of scientific equipment and resources including reagents, stocks of frozen sells, animal samples including acquisition, documentation, and storage.

• Program and use computers to store, process, and analyze data
• Monitor operational procedures in technical environments to ensure conformance to standards
• Interpret research or operational data
• Record research or operational data
• Develop new automated differentiation protocols for iPSC lines to differentiate into disease-relevant cell types like neurons, astrocytes, and microglia
• Develop image analysis routines to analyze the effects of disease-associated mutations on cellular phenotypes